Anti-IKB beta Rabbit mAb [54L41M87]

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Anti-IKB beta Rabbit mAb [54L41M87]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R012791
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-IKB beta Rabbit mAb [54L41M87]

All lanes: R012791 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 3: SW620 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-IKB beta Rabbit mAb [54L41M87]

Sample: Paraformaldehyde-fixed, paraffin embedded human hepatoma cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R012791 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-IKB beta Rabbit mAb [54L41M87]

Sample: Paraformaldehyde-fixed, paraffin embedded human breast cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R012791 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

产品信息
说明书
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Product Name	Anti-IKB beta Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), ELISA
Dilution	WB 1:1,000~1:2,000; IHC-P 1:100~1:200; IF 1:100~1:200
Reactivity	Mouse, Rat, Human
Host	Rabbit
Clonality	Monoclonal
Clone No.	54L41M87
Isotype	IgG
Label	Unconjugated
Immunogen	A synthesized peptide derived from human IKB beta
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	I kappa B beta, I-kappa-B-beta, IkappaBbeta, IKB beta, IkB-B, IkB-beta, IKBB, IKBB_HUMAN, IkBbeta, NF kappa BIB, NF-kappa-B inhibitor beta, NF-kappa-BIB, Nfkbib, Thyroid receptor interacting protein 9, Thyroid receptor-interacting protein 9, TR interacting protein 9, TR-interacting protein 9, TRIP-9, TRIP9.
Molecular weight	Calculated MW: 48 kDa; Observed MW: 48 kDa
Uniprot ID	Q15653
Gene ID	4793
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-IKB beta Rabbit mAb [54L41M87] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2011]

Background

The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2011]

Cellular Location	Cytoplasm. Nucleus.

Tissue Location	Expressed in all tissues examined.

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R012791 Anti-IKB beta Rabbit mAb [54L41M87]

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