Western blot analysis of VEGFD in Raw264.7 lysates using VEGFD antibody.

Western blot analysis of VEGFD in rat Brain, C6, Hela lysates using VEGFD antibody.

Immunocytochemistry analysis of VEGFD (green) in Hela using VEGFD antibody and DAPI (blue)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using VEGFD antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunohistochemistry - Anti-VEGFD Rabbit mAb [10M31K47]

Sample: Paraformaldehyde-fixed, paraffin embedded human renal carcinoma tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R012519 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunofluorescence - Anti-VEGFD Rabbit mAb [10M31K47]

Sample: Hela cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R012519 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Western Blot - Anti-VEGFD Rabbit mAb [10M31K47]

All lanes: R012519 at 1:3,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: C2C12 (Mouse myoblasts epithelial cell) whole cell lysates

Lane 5: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates

Lane 6: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 40 kDa

Observed band size: 40 kDa

Developed using the ECL technique (Cat. No. SQ201).