Anti-BAP1 Rabbit mAb [36K69K48]

抗体系列

Anti-BAP1 Rabbit mAb [36K69K48]

Purified Recombinant Rabbit Monoclonal Antibody

一键复制产品信息

货号	规格	价格
R012277
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

立即购买

Western Blot - Anti-BAP1 Rabbit mAb [36K69K48]

All lanes: R012277 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: A431 (Human epidermoid teratoma cell line) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 80 kDa

Observed band size: 95 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-BAP1 Rabbit mAb [36K69K48]

Sample: Paraformaldehyde-fixed, paraffin embedded rat testis tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R012277 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-BAP1 Rabbit mAb [36K69K48]

Sample: Paraformaldehyde-fixed, paraffin embedded human ovarian cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R012277 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-BAP1 Rabbit mAb [36K69K48]

Sample: Paraformaldehyde-fixed, paraffin embedded mouse lung tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R012277 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

产品信息
说明书
已发表文献

Product Name	Anti-BAP1 Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), ELISA
Dilution	WB 1:1,000~1:2,000; IHC-P 1:100~1:200; IF 1:100~1:200
Reactivity	Mouse, Rat, Human
Host	Rabbit
Clonality	Monoclonal
Clone No.	36K69K48
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant protein of human BAP1
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	BAP 1,Bap1,BAP1_HUMAN,BRCA 1 associated protein 1,BRCA1 associated protein 1,BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase),BRCA1-associated protein 1,Cerebral protein 13,Cerebral protein 6,DKFZp686N04275,FLJ35406,FLJ37180,HUCEP 13,Hucep 6,HUCEP13,Hucep6,KIAA0272,TPDS,Ubiquitin carboxy terminal hydrolase,Ubiquitin carboxyl terminal hydrolase BAP 1,Ubiquitin carboxyl terminal hydrolase BAP1,Ubiquitin carboxyl-terminal hydrolase BAP1,UCHL2.
Molecular weight	Calculated MW: 80 kDa; Observed MW: 95 kDa
Uniprot ID	Q92560
Gene ID	8314
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-BAP1 Rabbit mAb [36K69K48] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This gene belongs to the ubiquitin C-terminal hydrolase subfamily of deubiquitinating enzymes that are involved in the removal of ubiquitin from proteins. The encoded enzyme binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. Germline mutations in this gene may be associated with tumor predisposition syndrome (TPDS), which involves increased risk of cancers including malignant mesothelioma, uveal melanoma and cutaneous melanoma. [provided by RefSeq, May 2013]

Background

This gene belongs to the ubiquitin C-terminal hydrolase subfamily of deubiquitinating enzymes that are involved in the removal of ubiquitin from proteins. The encoded enzyme binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. Germline mutations in this gene may be associated with tumor predisposition syndrome (TPDS), which involves increased risk of cancers including malignant mesothelioma, uveal melanoma and cutaneous melanoma. [provided by RefSeq, May 2013]

Cellular Location	Cytoplasm. Nucleus. Mainly nuclear. Binds to chromatin.

Tissue Location	Highly expressed in testis, placenta and ovary. Expressed in breast.

说明书下载

R012277 Anti-BAP1 Rabbit mAb [36K69K48]

总篇数：加载中...

总影响因子：加载中...

最高影响因子：加载中...

加载中...

没有更多数据

产品中心

联系雅酶

上海市闵行区新骏环路760号1号楼3层

info@epizyme.cn

400-058-8030

技术支持(电话/微信)：

张老师 18217238522

罗老师 15021027255

李老师 18301721288

如有任何关于雅酶产品问题
欢迎来信咨询

雅酶微信公众号

抗体

蛋白提取与定量

蛋白电泳

蛋白免疫印迹

蛋白表达纯化与分离

蛋白质

ELISA试剂盒

核酸相关实验

微生物培养

设备与耗材

细胞培养

网站首页

产品中心

抗体

蛋白提取与定量

蛋白电泳

蛋白免疫印迹

蛋白表达纯化与分离

蛋白质

细胞培养

ELISA试剂盒

核酸相关实验

微生物培养

电泳成像设备与耗材

技术支持

活动资讯

联系我们

抗体系列