Anti-Phospho-MEK1 (Ser298) Rabbit mAb [36M99L32]

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Anti-Phospho-MEK1 (Ser298) Rabbit mAb [36M99L32]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R012258
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-Phospho-MEK1 (Ser298) Rabbit mAb [36M99L32]

All lanes: R012258 at 1:1,000 dilution

Lane 1: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 2: SCC-9 (Human tongue squamous carcinoma epithelial cell) whole cell lysates

Lane 3: SW620 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: C2C12 (Mouse myoblasts epithelial cell) whole cell lysates

Lane 5: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates

Lane 6: Balb/c mouse brain whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-Phospho-MEK1 (Ser298) Rabbit mAb [36M99L32]

Sample: HeLa cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R012258 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Product Name	Anti-Phospho-MEK1 (Ser298) Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	ELISA, WB, IF (Cell)/ICC
Dilution	WB 1:1,000~1:2,000; IF 1:100~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Clone No.	36M99L32
Isotype	IgG
Label	Unconjugated
Immunogen	A synthetic phosphopeptide corresponding to residues surrounding Ser298 of human MEK1
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	Dual specificity mitogen activated protein kinase kinase 1, Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAP kinase/Erk kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK 1, Mek1, MEKK1, Mitogen activated protein kinase kinase 1, MKK 1, MKK1, MP2K1_HUMAN, PRKMK1, Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1), Protein kinase mitogen activated, kinase 1, protein kinase mitogen-activated kinase 1.
Molecular weight	Calculated MW: 43 kDa; Observed MW: 43 kDa
Uniprot ID	Q02750
Gene ID	5604
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Phospho-MEK1 (Ser298) Rabbit mAb [36M99L32] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

Background

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

Tissue Location	Widely expressed, with extremely low levels in brain.

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R012258 Anti-Phospho-MEK1 (Ser298) Rabbit mAb [36M99L32]

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