Anti-Phospho-BRAF (Thr401) Rabbit mAb [86K47L17]

抗体系列

Anti-Phospho-BRAF (Thr401) Rabbit mAb [86K47L17]

Purified Recombinant Rabbit Monoclonal Antibody

一键复制产品信息

货号	规格	价格
R012011
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

立即购买

Western Blot - Anti-Phospho-BRAF (Thr401) Rabbit mAb [86K47L17]

All lanes: R012011 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: 293T (Human embryonic kidney cell) whole cell lysates

Lane 5: K562 (Human chronic myeloid leukemia cell) whole cell lysates

Lane 6: U87 (Human malignant glioblastoma epithelial cells) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 84 kDa

Observed band size: 84 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-Phospho-BRAF (Thr401) Rabbit mAb [86K47L17]

Sample: HeLa cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R012011 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Product Name	Anti-Phospho-BRAF (Thr401) Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF (Cell)/ICC, LISA
Dilution	WB 1:1,000~1:2,000; IF 1:100~1:200
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Clone No.	86K47L17
Isotype	IgG
Label	Unconjugated
Immunogen	A synthetic phosphopeptide corresponding to residues surrounding Thr401 of human B Raf
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	FLJ95109; 94 kDa B raf protein; B raf 1; B raf; B Raf proto oncogene serine threonine protein kinase; B Raf proto oncogene, serine/threonine kinase; B RAF1; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRAF 1; BRAF; BRAF_HUMAN; BRAF1; cRmil; MGC126806; MGC138284; Murine sarcoma viral (v-raf) oncogene homolog B1; Murine sarcoma viral v raf oncogene homolog B1; NS7; Oncogen BRAF; oncogene BRAF1; p94; Proto-oncogene B-Raf; Proto-oncogene c-Rmil; Raf; RAFB 1; RAFB1; RMIL; Serine/threonine-protein kinase B-raf; v raf murine sarcoma viral oncogene homolog B; v raf murine sarcoma viral oncogene homolog B1; v-Raf murine sarcoma viral oncogene homolog B1.
Molecular weight	Calculated MW: 84 kDa; Observed MW: 84 kDa
Uniprot ID	P15056
Gene ID	673
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Phospho-BRAF (Thr401) Rabbit mAb [86K47L17] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]

Background

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]

Cellular Location	Nucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.

Tissue Location	Brain and testis.

说明书下载

R012011 Anti-Phospho-BRAF (Thr401) Rabbit mAb [86K47L17]

总篇数：加载中...

总影响因子：加载中...

最高影响因子：加载中...

加载中...

没有更多数据

产品中心

联系雅酶

上海市闵行区新骏环路760号1号楼3层

info@epizyme.cn

400-058-8030

技术支持(电话/微信)：

张老师 18217238522

罗老师 15021027255

李老师 18301721288

如有任何关于雅酶产品问题
欢迎来信咨询

雅酶微信公众号

抗体

蛋白提取与定量

蛋白电泳

蛋白免疫印迹

蛋白表达纯化与分离

蛋白质

ELISA试剂盒

核酸相关实验

微生物培养

设备与耗材

细胞培养

网站首页