Anti-Phospho-CDC6 (Ser106) Rabbit mAb [50K60L33]

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Anti-Phospho-CDC6 (Ser106) Rabbit mAb [50K60L33]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R011720
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-Phospho-CDC6 (Ser106) Rabbit mAb [50K60L33]

All lanes: R011720 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: 293T (Human embryonic kidney cell) whole cell lysates

Lane 5: K562 (Human chronic myeloid leukemia cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 63 kDa

Observed band size: 63 kDa

Developed using the ECL technique (Cat. No. SQ201).

Product Name	Anti-Phospho-CDC6 (Ser106) Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, ELISA
Dilution	WB 1:1,000~1:2,000
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Clone No.	50K60L33
Isotype	IgG
Label	Unconjugated
Immunogen	A synthetic phosphopeptide corresponding to residues surrounding Ser106 of human Cdc6
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	CDC18L; CDC6; Cell division control protein 6 homolog; CDC6-related protein; Cdc18-related protein; p62(cdc6); HsCdc18; HsCDC6.
Molecular weight	Calculated MW: 63 kDa; Observed MW: 63 kDa
Uniprot ID	Q99741
Gene ID	990
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Phospho-CDC6 (Ser106) Rabbit mAb [50K60L33] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008]

Background

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008]

Cellular Location	Nucleus.Cytoplasm.The protein is nuclear in G1 and cytoplasmic in S-phase cells (PubMed:9566895).

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R011720 Anti-Phospho-CDC6 (Ser106) Rabbit mAb [50K60L33]

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