Anti-Phospho-delta 1 Catenin (Thr916) Rabbit mAb [12K71L79]

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Anti-Phospho-delta 1 Catenin (Thr916) Rabbit mAb [12K71L79]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R011484
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-Phospho-delta 1 Catenin (Thr916) Rabbit mAb [12K71L79]

All lanes: R011484 at 1:3,000 dilution

Lane 1: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 2: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates

Lane 3: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 108 kDa

Observed band size: 90-120 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-Phospho-delta 1 Catenin (Thr916) Rabbit mAb [12K71L79]

Sample: Caco-2 cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R011484 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Immunohistochemistry - Anti-Phospho-delta 1 Catenin (Thr916) Rabbit mAb [12K71L79]

Sample: Paraformaldehyde-fixed, paraffin embedded human breast cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R011484 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-Phospho-delta 1 Catenin (Thr916) Rabbit mAb [12K71L79]

Sample: Paraformaldehyde-fixed, paraffin embedded human gastric cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R011484 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

产品信息
说明书
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Product Name	Anti-Phospho-delta 1 Catenin (Thr916) Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), IF (Cell)/ICC, ELISA
Dilution	WB 1:1,000~1:3,000; IHC-P 1:100~1:200; IF 1:100~1:200
Reactivity	Human, Rat
Host	Rabbit
Clonality	Monoclonal
Clone No.	12K71L79
Isotype	IgG
Label	Unconjugated
Immunogen	A synthetic phosphopeptide corresponding to residues surrounding Thr916 of human delta 1 Catenin/CAS
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	CTND1_HUMAN; Catenin delta-1; delta 1 Catenin; KIAA0384; Cadherin-associated Src substrate(CAS); p120 catenin(p120(ctn)); p120(cas); CAS; p120; BCDS2; P120CAS; P120CTN; p120(CTN).
Molecular weight	Calculated MW: 108 kDa; Observed MW: 90-120 kDa
Uniprot ID	O60716
Gene ID	1500
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Phospho-delta 1 Catenin (Thr916) Rabbit mAb [12K71L79] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This gene encodes a member of the Armadillo protein family, which function in adhesion between cells and signal transduction. Multiple translation initiation codons and alternative splicing result in many different isoforms being translated. Not all of the full-length natures of the described transcript variants have been determined. Read-through transcription also exists between this gene and the neighboring upstream thioredoxin-related transmembrane protein 2 (TMX2) gene. [provided by RefSeq, Dec 2010]

Background

This gene encodes a member of the Armadillo protein family, which function in adhesion between cells and signal transduction. Multiple translation initiation codons and alternative splicing result in many different isoforms being translated. Not all of the full-length natures of the described transcript variants have been determined. Read-through transcription also exists between this gene and the neighboring upstream thioredoxin-related transmembrane protein 2 (TMX2) gene. [provided by RefSeq, Dec 2010]

Cellular Location	Cell junction Adherens junction Cytoplasm Nucleus Cell membrane Cell junction Interaction with GLIS2 promotes nuclear translocation (By similarity). Detected at cell-cell contacts (PubMed:15240885, PubMed:17047063). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm (PubMed:17047063). CDH1 enhances cell membrane localization (PubMed:15240885). Localizes to cell-cell contacts as keratinocyte differentiation progresses (By similarity). Isoform 1A Nucleus Isoform 2A Nucleus Isoform 3A Nucleus Isoform 4A Cytoplasm Isoform 1AB Cytoplasm

Cellular Location

Cell junction Adherens junction Cytoplasm Nucleus Cell membrane Cell junction Interaction with GLIS2 promotes nuclear translocation (By similarity). Detected at cell-cell contacts (PubMed:15240885, PubMed:17047063). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm (PubMed:17047063). CDH1 enhances cell membrane localization (PubMed:15240885). Localizes to cell-cell contacts as keratinocyte differentiation progresses (By similarity). Isoform 1A Nucleus Isoform 2A Nucleus Isoform 3A Nucleus Isoform 4A Cytoplasm Isoform 1AB Cytoplasm

Tissue Location	Expressed in vascular endothelium. Melanocytes and melanoma cells primarily express the long isoform 1A, whereas keratinocytes express shorter isoforms, especially 3A. The shortest isoform 4A, is detected in normal keratinocytes and melanocytes, and generally lost from cells derived from squamous cell carcinomas or melanomas. The C-terminal alternatively spliced exon B is present in the p120ctn transcripts in the colon, intestine and prostate, but lost in several tumor tissues derived from these organs.

Tissue Location

Expressed in vascular endothelium. Melanocytes and melanoma cells primarily express the long isoform 1A, whereas keratinocytes express shorter isoforms, especially 3A. The shortest isoform 4A, is detected in normal keratinocytes and melanocytes, and generally lost from cells derived from squamous cell carcinomas or melanomas. The C-terminal alternatively spliced exon B is present in the p120ctn transcripts in the colon, intestine and prostate, but lost in several tumor tissues derived from these organs.

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R011484 Anti-Phospho-delta 1 Catenin (Thr916) Rabbit mAb [12K71L79]

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