Anti-SNAP25 Rabbit mAb [97M21K98]

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Anti-SNAP25 Rabbit mAb [97M21K98]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R011310
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-SNAP25 Rabbit mAb [97M21K98]

All lanes: R011310 at 1:1,000 dilution

Lane 1: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates

Lane 2: Mouse brain whole tissue lysates

Lane 3: Rat brain whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 23 kDa

Observed band size: 25 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-SNAP25 Rabbit mAb [97M21K98]

Sample: HeLa cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R011310 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Immunohistochemistry - Anti-SNAP25 Rabbit mAb [97M21K98]

Sample: Paraformaldehyde-fixed, paraffin embedded rat brain tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R011310 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-SNAP25 Rabbit mAb [97M21K98]

Sample: Paraformaldehyde-fixed, paraffin embedded mouse brain tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R011310 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

产品信息
说明书
已发表文献

Product Name	Anti-SNAP25 Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), IF (Cell)/ICC, ELISA
Dilution	WB 1:1,000~1:2,000; IHC-P 1:100~1:200; IF 1:100~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Clone No.	97M21K98
Isotype	IgG
Label	Unconjugated
Immunogen	A synthesized peptide derived from human SNAP25
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	SNAP; SNAP25; Synaptosomal-associated protein 25; SNAP-25; Super protein; Synaptosomal-associated 25 kDa protein; SUP.
Molecular weight	Calculated MW: 23 kDa; Observed MW: 25 kDa
Uniprot ID	P60880
Gene ID	6616
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-SNAP25 Rabbit mAb [97M21K98] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Background

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Cellular Location	Cytoplasm.Perinuclear region.Cell membrane.Lipid-anchor.Synapse.Synaptosome.Photoreceptor inner segment.Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. Colocalizes with KCNB1 at the cell membrane (By similarity). Colocalizes with PLCL1 at the cell membrane (By similarity).

Tissue Location	Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.

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R011310 Anti-SNAP25 Rabbit mAb [97M21K98]

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