Anti-Phospho-FOXO3A (Ser253) Rabbit mAb [42K60K09]

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Anti-Phospho-FOXO3A (Ser253) Rabbit mAb [42K60K09]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R010946
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-Phospho-FOXO3A (Ser253) Rabbit mAb [42K60K09]

All lanes: R010946 at 1:1,000 dilution

Lane 1: Hel (Human erythroLeukemia suspension cell) whole cell lysates

Lane 2: Ball-1 (Human B lymphocyte acute leukemia cell) whole cell lysates

Lane 3: K562 (Human chronic myeloid leukemia cell) whole cell lysates

Lane 4: U87 (Human malignant glioblastoma epithelial cells) whole cell lysates

Lane 5: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates

Lane 6: Mouse embryo whole tissue lysates

Lane 7: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lane 8: Rat spleen whole tissue lysates

Lane 9: Rat lymphoid whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 71 kDa

Observed band size: 82 kDa

Developed using the ECL technique (Cat. No. SQ201).

Product Name	Anti-Phospho-FOXO3A (Ser253) Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, ELISA
Dilution	WB 1:1,000~1:2,000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Clone No.	42K60K09
Isotype	IgG
Label	Unconjugated
Immunogen	A synthetic phosphopeptide corresponding to residues surrounding Ser253 of human FOXO3A
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	AF6q21; AF6q21 protein; DKFZp781A0677; FKHR2; FKHRL 1; FKHRL1; FKHRL1P2; Forkhead(Drosophila) homolog(rhabdomyosarcoma) like 1; Forkhead box O3A; Forkhead box protein O3A; Forkhead Drosophila homolog of in rhabdomyosarcoma like 1; Forkhead in rhabdomyosarcoma like 1; FOX O3A; FOXO2; MGC12739; MGC31925; FOXO3_HUMAN.
Molecular weight	Calculated MW: 71 kDa; Observed MW: 82 kDa
Uniprot ID	O43524
Gene ID	2309
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Phospho-FOXO3A (Ser253) Rabbit mAb [42K60K09] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Cellular Location	Cytoplasm Cytosol Nucleus Mitochondrion matrix Mitochondrion outer membrane Peripheral membrane protein Cytoplasmic side Retention in the cytoplasm contributes to its inactivation (PubMed:10102273, PubMed:15084260, PubMed:16751106). Translocates to the nucleus upon oxidative stress and in the absence of survival factors (PubMed:10102273, PubMed:16751106). Translocates from the cytosol to the nucleus following dephosphorylation in response to autophagy-inducing stimuli (By similarity). Translocates in a AMPK-dependent manner into the mitochondrion in response to metabolic stress (PubMed:23283301, PubMed:29445193). Serum deprivation increases localization to the nucleus, leading to activate expression of SOX9 and subsequent chondrogenesis (By similarity).

Cellular Location

Cytoplasm Cytosol Nucleus Mitochondrion matrix Mitochondrion outer membrane Peripheral membrane protein Cytoplasmic side Retention in the cytoplasm contributes to its inactivation (PubMed:10102273, PubMed:15084260, PubMed:16751106). Translocates to the nucleus upon oxidative stress and in the absence of survival factors (PubMed:10102273, PubMed:16751106). Translocates from the cytosol to the nucleus following dephosphorylation in response to autophagy-inducing stimuli (By similarity). Translocates in a AMPK-dependent manner into the mitochondrion in response to metabolic stress (PubMed:23283301, PubMed:29445193). Serum deprivation increases localization to the nucleus, leading to activate expression of SOX9 and subsequent chondrogenesis (By similarity).

Tissue Location	Ubiquitous.

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R010946 Anti-Phospho-FOXO3A (Ser253) Rabbit mAb [42K60K09]

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