Anti-STAT5a/STAT5b Rabbit mAb [69M23K46]

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Anti-STAT5a/STAT5b Rabbit mAb [69M23K46]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R010941
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-STAT5a/STAT5b Rabbit mAb [69M23K46]

All lanes: R010941 at 1:1,000 dilution

Lane 1: K562 (Human chronic myeloid leukemia cell) whole cell lysates

Lane 2: U87 (Human malignant glioblastoma epithelial cell) whole cell lysates

Lane 3: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates

Lane 4: Mouse spleen whole tissue lysates

Lane 5: Mouse embryo-like whole tissue lysates

Lane 6: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lane 7: Rat spleen whole tissue lysates

Lane 8: Rat lymphoid whole tissue lysates

Lane 9: Rat testicular whole tissue lysates

Lane 10: Rat eyeball whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 91 kDa

Observed band size: 91 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-STAT5a/STAT5b Rabbit mAb [69M23K46]

Sample: HeLa cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R010941 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Immunohistochemistry - Anti-STAT5a/STAT5b Rabbit mAb [69M23K46]

Sample: Paraformaldehyde-fixed, paraffin embedded human breast cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R010941 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

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Product Name	Anti-STAT5a/STAT5b Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), IF (Cell)/ICC, ELISA
Dilution	WB 1:1,000~1:2,000; IHC-P 1:100~1:200; IF 1:100~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Clone No.	69M23K46
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant protein of human STAT5A
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	signal transducers and activators of transduction 5; MGF; Signal Transducer and Activator of Transcription 5A; STAT 5; STAT 5A; STAT 5B; STAT5A; STAT5B; Transcription factor STAT5A; STA5B_HUMAN; STA5A_HUMAN.
Molecular weight	Calculated MW: 91 kDa; Observed MW: 91 kDa
Uniprot ID	P42230
Gene ID	6776
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-STAT5a/STAT5b Rabbit mAb [69M23K46] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]

Cellular Location	Cytoplasm. Nucleus. Note=Mostly cytoplasmic during pregnancy, whereas it becomes predominantly nuclear during early lactation. It is translocated into the nucleus in response to phosphorylation.

Tissue Location	Expressed in heart, lung, and weakly in muscle.

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R010941 Anti-STAT5a/STAT5b Rabbit mAb [69M23K46]

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