Purified Rabbit Monoclonal Antibody
| 货号 | 规格 | 价格 |
|---|---|---|
| R010721 | ||
| 20µL | ¥588.00 | |
| 50µL | ¥1080.00 | |
| 100µL | ¥1780.00 |
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| Product Name | Anti-eIF4E Rabbit mAb |
|---|---|
| Description | Purified Rabbit Monoclonal Antibody |
Application
|
ICC/IF (Cell), IP, ELISA, WB |
| Dilution | WB 1:500~1:1,000; IF 1:50~1:200; IP 1:20 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | 60M83K91 |
| Isotype | IgG |
| Label | Unconjugated |
| Immunogen | A synthetic peptide of human eIF4E |
| Format | Buffer System: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA Purification: Affinity Purified. |
| Synonyms | EIF4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein. |
|---|---|
| Molecular weight | Calculated MW: 25 kDa; Observed MW: 25 kDa |
| Uniprot ID | P06730 |
| Gene ID | 1977 |
| Storage | Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage. |
| Precautions | Anti-eIF4E antibody [60M83K91] is for research use only and not for use in diagnostic or therapeutic procedures. |
| Background | eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. |
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