Anti-Phospho-ERK1/2 (Thr202/Thr185) Rabbit mAb [47K36K34]

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Anti-Phospho-ERK1/2 (Thr202/Thr185) Rabbit mAb [47K36K34]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R010505
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-Phospho-ERK1/2 (Thr202/Thr185) Rabbit mAb [47K36K34]

All lanes: R010505 at 1:1,000 dilution

Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysates

Lane 3: Jurkat (human T lymphocytic leukemia cell) whole cell lysates

Lane 4: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysates

Lane 5: T24 (human bladder cancer epithelial cell) whole cell lysates

Lane 6: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysates

Lane 7: SW620 (human colorectal carcinoma epithelial cell) whole cell lysates

Lane 8: U2OS (human osteosarcoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 42 kDa, 44 kDa

Observed band size: 42 kDa, 44 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-Phospho-ERK1/2 (Thr202/Thr185) Rabbit mAb [47K36K34]

Sample: Paraformaldehyde-fixed, paraffin embedded rat stomach tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R010505 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-Phospho-ERK1/2 (Thr202/Thr185) Rabbit mAb [47K36K34]

Sample: Paraformaldehyde-fixed, paraffin embedded human colorectal carcinoma tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R010505 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

产品信息
说明书
已发表文献

Product Name	Anti-Phospho-ERK1/2 (Thr202/Thr185) Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	ELISA, WB, IHC-P/IF (Tissue-P)
Dilution	WB 1:1,000~1:2,000; IHC-P 1:200; IF 1:100~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Clone No.	47K36K34
Isotype	IgG
Label	Unconjugated
Immunogen	A synthetic phosphopeptide corresponding to residues surrounding Thr185 of human ERK2
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	MAPK1/MAPK3.
Molecular weight	Calculated MW: 42 kDa, 44 kDa; Observed MW: 42 kDa, 44 kDa
Uniprot ID	P27361 , P28482
Gene ID	5595 , 5594
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Phospho-ERK1/2 (Thr202/Thr185) antibody [47K36K34] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements.

Background

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements.

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R010505 Anti-Phospho-ERK1/2 (Thr202/Thr185) Rabbit mAb [47K36K34]

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