Immunocytochemistry analysis of Tissue Factor (green) in SKOV-3 using Tissue Factor antibody and DAPI (blue) .

Western blot analysis of Tissue Factor in A549, HL-60, U2OS lysates using Tissue Factor antibody.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Tissue Factor antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence - Anti-Tissue Factor Rabbit mAb [83M71K67]

Sample: A431 cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R010436 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Western Blot - Anti-Tissue Factor Rabbit mAb [83M71K67]

All lanes: R010436 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: U2OS (Human osteosarcoma epithelial cell) whole cell lysates

Lane 5: T24 (Human bladder cancer epithelial cell) whole cell lysates

Lane 6: SCC-9 (Human tongue squamous carcinoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 33 kDa

Observed band size: 45 kDa

Developed using the ECL technique (Cat. No. SQ201).