Immunohistochemistry - Anti-ARHGAP25 Rabbit mAb [10M49L39]

Sample: Paraformaldehyde-fixed, paraffin embedded rat stomach tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R010395 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Western Blot - Anti-ARHGAP25 Rabbit mAb [10M49L39]

All lanes: R010395 at 1:1,000 dilution

Lane 1: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates

Lane 2: Mouse brain whole tissue lysates

Lane 3: Rat heart whole tissue lysates

Lane 4: Rat liver whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

Developed using the ECL technique (Cat. No. SQ201).

Western Blot - Anti-ARHGAP25 Rabbit mAb [10M49L39]

All lanes: R010395 at 1:1,000 dilution

Lane 1: K562 (Human chronic myeloid leukemia cell) whole cell lysates

Lane 2: Mouse heart whole tissue lysates

Lane 3: Mouse brain whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

Developed using the ECL technique (Cat. No. SQ201).