Western Blot - Anti-Caveolin 1 Rabbit mAb [23M64M06]

All lanes: R010327 at 1:4,000 dilution

Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: A549 (human non-small cell lung cancer epithelial cell) whole cell lysates

Lane 3: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: T24 (human bladder cancer epithelial cell) whole cell lysatess

Lane 5: SCC-9 (human tongue squamous carcinoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-Caveolin 1 Rabbit mAb [23M64M06]

Sample: Paraformaldehyde-fixed, paraffin embedded rat lung tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R010327 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-Caveolin 1 Rabbit mAb [23M64M06]

Sample: Paraformaldehyde-fixed, paraffin embedded human breast carcinoma tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R010327 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-Caveolin 1 Rabbit mAb [23M64M06]

Sample: Paraformaldehyde-fixed, paraffin embedded mouse uterus tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R010327 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Western Blot - Anti-Caveolin 1 Rabbit mAb [23M64M06]

All lanes: R010327 at 1:1,000 dilution

Cytosolic and membrane protein were extracted using  Membrane and Cytosol Protein Extraction Kit (Cat. No. PC202), 

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Developed using the ECL technique (Cat. No. SQ201).