Anti-RAP1A/B Rabbit mAb [36M99L04]

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Anti-RAP1A/B Rabbit mAb [36M99L04]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R010303
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-RAP1A/B Rabbit mAb [36M99L04]

All lanes: R010303 at 1:5,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: Huh1 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 4: 293T (Human embryonic kidney cell) whole cell lysates

Lane 5: U87 (Human malignant glioblastoma epithelial cells) whole cell lysates

Lane 6: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates

Lane 7: Mouse heart whole tissue lysates

Lane 8: Mouse liver whole tissue lysates

Lane 9: Mouse brain whole tissue lysates

Lane 10: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lane 11: Rat muscle whole tissue lysates

Lane 12: Rat brain whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-RAP1A/B Rabbit mAb [36M99L04]

Sample: HeLa cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R010303 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

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Product Name	Anti-RAP1A/B Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF (Cell)/ICC, ELISA
Dilution	WB 1:1,000~1:5,000; IF 1:100~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Clone No.	36M99L04
Isotype	IgG
Label	Unconjugated
Immunogen	A synthesized peptide derived from human RAP1A/B
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	C21KG; G-22K; GTP-binding protein smg p21A; GTP-binding protein smg p21B; KREV1; OK/SW-cl.11; Ras-related protein Krev-1; Ras-related protein Rap-1A; Ras-related protein Rap-1b; RAP1A; RAP1B; RAP1.
Molecular weight	Calculated MW: 21 kDa; Observed MW: 21 kDa
Uniprot ID	P61224
Gene ID	5908
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-RAP1A/B Rabbit mAb [36M99L04] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Background

This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Cellular Location	Cell membrane; Lipid-anchor. Cytoplasm. Cytoplasm › perinuclear region. Cell junction By similarity. Early endosome By similarity.

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R010303 Anti-RAP1A/B Rabbit mAb [36M99L04]

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