Western Blot - Anti-beta Catenin Rabbit mAb [41L48K19]

All lanes: R010292 at 1:1,000 dilution

Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 3: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysates

Lane 4: A549 ((human lung carcinoma epithelial cell) whole cell lysates

Lane 5: 293T (human embryonic kidney cell) whole cell lysates

Lane 6: MSC (human mesenchymal stem cell) whole cell lysates

Lane 7: Balb/c mouse brain whole tissue lysates

Lane 8: Balb/c mouse lung whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 85 kDa

Observed band size: 85-95 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-beta Catenin Rabbit mAb [41L48K19]

Sample: Paraformaldehyde-fixed, paraffin embedded human cervical cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R010292 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-beta Catenin Rabbit mAb [41L48K19]

Sample: Paraformaldehyde-fixed, paraffin embedded human pancreatic cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R010292 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunofluorescence - Anti-beta Catenin Rabbit mAb [41L48K19]

Sample: Paraformaldehyde-fixed, paraffin embedded human colorectal carcinoma tissue

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibodies: R010292 at 1:100 dilution and Anti-ZO-1 Mouse mAb [24D02C18] (Cat. No. M900002) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (CY3) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Immunofluorescence - Anti-beta Catenin Rabbit mAb [41L48K19]

Sample: A431 cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R010292 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (CY3) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).