Western Blot - Anti-POLDIP2 Rabbit mAb [40M32K51]

All lanes: R010241 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lane 4: Rat liver whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 42 kDa

Observed band size: 38 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-POLDIP2 Rabbit mAb [40M32K51]

Sample: Paraformaldehyde-fixed, paraffin embedded human hepatoma tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R010241 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-POLDIP2 Rabbit mAb [40M32K51]

Sample: Paraformaldehyde-fixed, paraffin embedded human cervical cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R010241 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.