Western Blot - Anti-Desmin Rabbit pAb

All lanes: P900004 at 1:1,000 dilution

Lane 1: RD (Human rhabdomyosarcoma cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-Desmin Rabbit pAb

Sample: Paraformaldehyde-fixed, paraffin embedded mouse skeletal muscle tissue

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: P900004 at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green)

Nuclei were stained with DAPI (shown in blue).

Immunofluorescence - Anti-Desmin Rabbit pAb

Sample: C2C12 cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: P900004 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (CY3) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

All lanes: Anti-Desmin Rabbit pAb at 1:1,000 dilution

Lane 1: RD cell Lysates

Lane 2: SCC-9 cell Lysates

Lane 3: NCI-H2347 cell Lysates

Lysates/proteins at 20 μg per lane.

Secondary Goat Anti-Rabbit IgG, (H L), Peroxidase conjugated (LF102) at 1:5,000 dilution.

Observed band size: 52 kDa

Blocking/Dilution buffer: 1×PBST.

Arrow indicated the observed band.

Note: mRNA level was aquired from Protein Atlas database.

Immunohistochemical analysis of paraffin embedded gastric cancer tissue using Anti-Desmin Rabbit pAb. Antigen repair using EDTA antigen repair solution and blocking with 5% BSA for half 0.5 hour. Samples were incubated with primary antibody (1:1,000) overnight at 4℃. A undiluted HRP-labeled anti-rabbit IgG was used as the secondary antibody for 0.5 hour.

Immunohistochemical analysis of paraffin embedded colon cancer tissue using Anti-Desmin Rabbit pAb. Antigen repair using EDTA antigen repair solution and blocking with 5% BSA for half 0.5 hour. Samples were incubated with primary antibody (1:1000) overnight at 4℃. A undiluted HRP-labeled anti-rabbit IgG was used as the secondary antibody for 0.5 hour.