Anti-Phospho-Cyclin D1 (Thr286) Rabbit pAb

抗体系列

Anti-Phospho-Cyclin D1 (Thr286) Rabbit pAb

Purified Rabbit Polyclonal Antibody

一键复制产品信息

货号	规格	价格
P108995
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

立即购买

Anti-Phospho-Cyclin D1 (Thr286) Rabbit pAb

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Cyclin D1-T286 pAb (P108995) at 1:1,000 dilution or Cyclin D1 antibody (P012243). NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% BSA.

Detection: ECL Kit.

Exposure time: 60s.

Immunohistochemistry analysis of paraffin-embedded Rat eye using Phospho-Cyclin D1-T286 Rabbit pAb (P108995) at dilution of 1:100 (40× lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Phospho-Cyclin D1-T286 Rabbit pAb (P108995) at dilution of 1:100 (40× lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse eye using Phospho-Cyclin D1-T286 Rabbit pAb (P108995) at dilution of 1:100 (40× lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Product Name	Anti-Phospho-Cyclin D1 (Thr286) Rabbit pAb
Description	Purified Rabbit Polyclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), ELISA
Dilution	WB 1:500~1:2,000; IHC-P 1:50~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Label	Unconjugated
Immunogen	A synthetic phosphorylated peptide around T286 of human Cyclin D1 (NP_444284.1).
Format	Affinity purified polyclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	BCL1; PRAD1; U21B31; D11S287E; Phospho-Cyclin D1-T286.
Molecular weight	Calculated MW: 34 kDa; Observed MW: 37 kDa
Uniprot ID	P24385
Gene ID	595
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Phospho-Cyclin D1 (Thr286) Rabbit pAb is for research use only and not for use in diagnostic or therapeutic procedures.

Background	The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers.

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers.

说明书下载

P108995 Anti-Phospho-Cyclin D1 (Thr286) Rabbit pAb

总篇数：加载中...

总影响因子：加载中...

最高影响因子：加载中...

加载中...

没有更多数据

产品中心

联系雅酶

上海市闵行区新骏环路760号1号楼3层

info@epizyme.cn

400-058-8030

技术支持(电话/微信)：

张老师 18217238522

罗老师 15021027255

李老师 18301721288

如有任何关于雅酶产品问题
欢迎来信咨询

雅酶微信公众号

抗体

蛋白提取与定量

蛋白电泳

蛋白免疫印迹

蛋白表达纯化与分离

蛋白质

ELISA试剂盒

核酸相关实验

微生物培养

设备与耗材

细胞培养

网站首页