Anti-Phospho-ABL1 (Tyr412) Rabbit pAb

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Anti-Phospho-ABL1 (Tyr412) Rabbit pAb

Purified Rabbit Polyclonal Antibody

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货号	规格	价格
P108836
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western blot analysis of lysates from C6 cells, using Phospho-c-Abl-Y412 Rabbit pAb (P108836). C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% BSA.

Detection: ECL Kit.

Exposure time: 3s.

Immunohistochemistry analysis of paraffin-embedded Human appendix using Phospho-c-Abl-Y412 Rabbit pAb (P108836) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-c-Abl-Y412 Rabbit pAb (P108836) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse liver using Phospho-c-Abl-Y412 Rabbit pAb (P108836) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Product Name	Anti-Phospho-ABL1 (Tyr412) Rabbit pAb
Description	Purified Rabbit Polyclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), ELISA
Dilution	WB 1:500~1:2,000; IHC-P 1:50~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Label	Unconjugated
Immunogen	A synthetic phosphorylated peptide around Y412 of human ABL1 (NP_005148.2).
Format	Affinity purified polyclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	ABL; JTK7; p150; c-ABL; v-abl; CHDSKM; c-ABL1; BCR-ABL; bcr/abl; Phospho-c-Abl-Y412.
Molecular weight	Calculated MW: 123 kDa; Observed MW: 123 kDa
Uniprot ID	P00519
Gene ID	25
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Phospho-ABL1 (Tyr412) Rabbit pAb is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons.

Background

This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons.

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P108836 Anti-Phospho-ABL1 (Tyr412) Rabbit pAb

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