Anti-Phospho-VASP (Ser157) Rabbit pAb

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Anti-Phospho-VASP (Ser157) Rabbit pAb

Purified Rabbit Polyclonal Antibody

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货号	规格	价格
P108794
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western blot analysis of lysates from HEL cells using Phospho-VASP-S157 Rabbit pAb (P108794) at 1:1,000 dilution. HEL cells were treated by Forskolin (20 uM) at 37℃ for 1 hour.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25 μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit.

Exposure time: 0.8s.

Immunoprecipitation analysis of 200 μg extracts of A-431 cells, using 3 μg Phospho-VASP-S157 pAb (P108794). Western blot was performed from the immunoprecipitate using Phospho-VASP-S157 pAb (P108794) at a dilution of 1:1000. A-431 cells were treated by Forskolin (10 uM) at 37℃ for 30 minutes after serum-starvation overnight.

Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-VASP-S157 Rabbit pAb (P108794) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat brain using Phospho-VASP-S157 Rabbit pAb (P108794) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse lung using Phospho-VASP-S157 Rabbit pAb (P108794) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Product Name	Anti-Phospho-VASP (Ser157) Rabbit pAb
Description	Purified Rabbit Polyclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), IP, ELISA
Dilution	WB 1:500~1:2,000; IHC-P 1:50~1:100; IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Label	Unconjugated
Immunogen	A synthetic phosphorylated peptide around S157 of human Phospho-VASP-S157 (NP_003361.1).
Format	Affinity purified polyclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	Phospho-VASP-S157.
Molecular weight	Calculated MW: 40 kDa; Observed MW: 50 kDa
Uniprot ID	P50552
Gene ID	7408
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Phospho-VASP (Ser157) Rabbit pAb is for research use only and not for use in diagnostic or therapeutic procedures.

Background	Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

Background

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

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P108794 Anti-Phospho-VASP (Ser157) Rabbit pAb

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