Anti-Phospho-ATP Citrate lyase (Ser455) Rabbit pAb

抗体系列

Anti-Phospho-ATP Citrate lyase (Ser455) Rabbit pAb

Purified Rabbit Polyclonal Antibody

一键复制产品信息

货号	规格	价格
P108489
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

立即购买

Anti-Phospho-ATP Citrate lyase (Ser455) Rabbit pAb

Western blot analysis of lysates from HeLa cells using Phospho-ACLY-S455 Rabbit pAb (P108489) at 1:400 dilution. HeLa cells were treated by Insulin (50 nM) at 37℃ for 30 minutes after serum-starvation overnight.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25 μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit.

Exposure time: 60s.

Western blot analysis of lysates from C6 cells using Phospho-ACLY-S455 Rabbit pAb (P108489) at 1:400 dilution. C6 cells were treated by Insulin (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25 μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit.

Exposure time: 60s.

Western blot analysis of lysates from NIH/3T3 cells using Phospho-ACLY-S455 Rabbit pAb (P108489) at 1:400 dilution. NIH/3T3 cells were treated by Insulin (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25 μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit.

Exposure time: 60s.

Immunoprecipitation analysis of 200 μg extracts of NIH/3T3 cells, using 3 μg Phospho-ACLY-S455 pAb (P108489). Western blot was performed from the immunoprecipitate using Phospho-ACLY-S455 pAb (P108489) at a dilution of 1:1000. NIH/3T3 cells were treated by Insulin (100 nM) at 37℃ for 10 minutes after serum-starvation overnight.

Immunohistochemistry analysis of paraffin-embedded Rat pancreas using Phospho-ACLY-S455 Rabbit pAb (P108489) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse brain using Phospho-ACLY-S455 Rabbit pAb (P108489) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using Phospho-ACLY-S455 Rabbit pAb (P108489) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

产品信息
说明书
已发表文献

Product Name	Anti-Phospho-ATP Citrate lyase (Ser455) Rabbit pAb
Description	Purified Rabbit Polyclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), IP, ELISA
Dilution	WB 1:100~1:500 ; IHC-P 1:50~1:100; IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Label	Unconjugated
Immunogen	A synthetic phosphorylated peptide around S455 of human ACLY (NP_001087.2).
Format	Affinity purified polyclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	ACL; ATPCL; CLATP; Phospho-ACLY-S455.
Molecular weight	Calculated MW: 121 kDa; Observed MW: 125 kDa
Uniprot ID	P53396
Gene ID	47
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Phospho-ATP Citrate lyase (Ser455) Rabbit pAb is for research use only and not for use in diagnostic or therapeutic procedures.

Background	ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.

Background

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.

说明书下载

P108489 Anti-Phospho-ATP Citrate lyase (Ser455) Rabbit pAb

总篇数：加载中...

总影响因子：加载中...

最高影响因子：加载中...

加载中...

没有更多数据

产品中心

联系雅酶

上海市闵行区新骏环路760号1号楼3层

info@epizyme.cn

400-058-8030

技术支持(电话/微信)：

张老师 18217238522

罗老师 15021027255

李老师 18301721288

如有任何关于雅酶产品问题
欢迎来信咨询

雅酶微信公众号

抗体

蛋白提取与定量

蛋白电泳

蛋白免疫印迹

蛋白表达纯化与分离

蛋白质

ELISA试剂盒

核酸相关实验

微生物培养

设备与耗材

细胞培养

网站首页

产品中心

抗体

蛋白提取与定量

蛋白电泳

蛋白免疫印迹

蛋白表达纯化与分离

蛋白质

细胞培养

ELISA试剂盒

核酸相关实验

微生物培养

电泳成像设备与耗材

技术支持

活动资讯

联系我们

抗体系列