Western blot analysis of various lysates using Phospho-Lamin A/C-S22 Rabbit pAb (P108482) at 1:2,000 dilution or Lamin A antibody (P100118). HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM/mL) at 37℃ for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM) at 37℃ for 20 hours.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% BSA.

Detection: ECL Enhanced Kit.

Exposure time: 1s.

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Phospho-Lamin A/C-S22 pAb (P108482). Western blot was performed from the immunoprecipitate using Phospho-Lamin A/C-S22 pAb (P108482) at a dilution of 1:1000. HeLa cells were treated by Paclitaxel (100 nM/mL) at 37℃ for 20 hours.