Anti-Phospho-IKB alpha (Ser32) Rabbit pAb

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Anti-Phospho-IKB alpha (Ser32) Rabbit pAb

Purified Rabbit Polyclonal Antibody

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货号	规格	价格
P108473
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Anti-Phospho-IKB alpha (Ser32) Rabbit pAb

Western blot analysis of lysates from HeLa cells, using Phospho-IκBα-S32 Rabbit pAb (P108473) or IκBα Rabbit pAb (P100832). HeLa cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% BSA.

Detection: ECL Kit.

Exposure time: 3min.

Western blot analysis of lysates from HeLa cells, using Phospho-IκBα-S32 Rabbit pAb (P108473) at 1:1,000 dilution. HeLa cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% BSA.

Detection: ECL Kit.

Exposure time: 90s.

Immunohistochemistry analysis of paraffin-embedded Rat brain using Phospho-IκBα-S32 Rabbit pAb (P108473) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-IκBα-S32 Rabbit pAb (P108473) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse brain using Phospho-IκBα-S32 Rabbit pAb (P108473) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunofluorescence analysis of C6 cells using Phospho-IκBα-S32 Rabbit pAb (P108473) at dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using Phospho-IκBα-S32 Rabbit pAb (P108473) at dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using Phospho-IκBα-S32 Rabbit pAb (P108473) at dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

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说明书
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Product Name	Anti-Phospho-IKB alpha (Ser32) Rabbit pAb
Description	Purified Rabbit Polyclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), IF (Cell)/ICC, ELISA
Dilution	WB 1:500~1:2,000; IHC-P 1:50~1:200; IF 1:50~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Label	Unconjugated
Immunogen	A synthetic phosphorylated peptide around S32 of human IκBα (NP_065390.1).
Format	Affinity purified polyclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	IKBA; MAD-3; NFKBI; EDAID2; Phospho-IκBα-S32.
Molecular weight	Calculated MW: 36 kDa; Observed MW: 39 kDa/41 kDa
Uniprot ID	P25963
Gene ID	4792
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Phospho-IKB alpha (Ser32) Rabbit pAb is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.

Background

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.

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P108473 Anti-Phospho-IKB alpha (Ser32) Rabbit pAb

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