Western blot analysis of lysates from HT-29 cells, using Phospho-cdc25C-S216 Rabbit pAb (P108472) at 1:400 dilution. HT-29 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit.

Exposure time: 90s.

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Phospho-cdc25C-S216 Rabbit pAb (P108472) at a dilution of 1:100 (40× lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Phospho-cdc25C-S216 Rabbit pAb (P108472) at a dilution of 1:100 (40× lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using Phospho-cdc25C-S216 Rabbit pAb (P108472) at a dilution of 1:100 (40× lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Phospho-cdc25C-S216 Rabbit pAb (P108472) at a dilution of 1:100 (40× lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Phospho-cdc25C-S216 Rabbit pAb (P108472) at a dilution of 1:100 (40× lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Phospho-cdc25C-S216 Rabbit pAb (P108472) at a dilution of 1:100 (40× lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.