Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using PP2A Catalytic α Rabbit pAb (P106982) at a dilution of 1:100 (40× lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human cervical cance tissue using PP2A Catalytic α Rabbit pAb (P106982) at a dilution of 1:100 (40× lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using PP2A Catalytic α Rabbit pAb (P106982) at a dilution of 1:100 (40× lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunofluorescence analysis of HeLa cells using PP2A Catalytic α Rabbit pAb (P106982) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using PP2A Catalytic α Rabbit pAb (P106982) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western blot analysis of various lysates, using PP2A Catalytic α Rabbit pAb (P106982) at 1:2,000 dilution.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit.

Exposure time: 10s.

Immunoprecipitation analysis of 200 μg extracts of 293T cells using 3 μg PP2A Catalytic α antibody (P106982). Western blot was performed from the immunoprecipitate using PP2A Catalytic α antibody (P106982) at a dilution of 1:1000.

Immunoprecipitation analysis of 500 μg extracts of Rat brain cells using 3 μg PP2A Catalytic α antibody (P106982). Western blot was performed from the immunoprecipitate using PP2A Catalytic α antibody (P106982) at a dilution of 1:1000.