Immunohistochemistry analysis of paraffin-embedded Rat kidney using [KO Validated] α-Catenin Rabbit pAb (P106396) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human vermiform appendix using [KO Validated] α-Catenin Rabbit pAb (P106396) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human stomach using [KO Validated] α-Catenin Rabbit pAb (P106396) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse testis using [KO Validated] α-Catenin Rabbit pAb (P106396) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Western blot analysis of various lysates using [KO Validated] α-Catenin Rabbit pAb (P106396) at 1:1,000 dilution.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit.

Exposure time: 60s.

Western blot analysis of lysates from wild type (WT) and α-Catenin knockout (KO) 293T cells, using [KO Validated] α-Catenin Rabbit pAb (P106396) at 1:1,000 dilution.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit.

Exposure time: 10s.

Immunoprecipitation analysis of 200 μg extracts of 293T cells using 3 μg α-Catenin antibody (P106396). Western blot was performed from the immunoprecipitate using α-Catenin antibody (P106396) at a dilution of 1:500.