[KO Validated] Anti-HuR/ELAVL1 Rabbit pAb

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[KO Validated] Anti-HuR/ELAVL1 Rabbit pAb

Purified Rabbit Polyclonal Antibody

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货号	规格	价格
P106333
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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[KO Validated] Anti-HuR/ELAVL1 Rabbit pAb

Immunohistochemistry analysis of paraffin-embedded Human esophagus using [KO Validated] HuR/ELAVL1 Rabbit pAb (P106333) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human stomach using [KO Validated] HuR/ELAVL1 Rabbit pAb (P106333) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse brain using [KO Validated] HuR/ELAVL1 Rabbit pAb (P106333) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunofluorescence analysis of U2OS cells using [KO Validated] HuR/ELAVL1 Rabbit pAb (P106333) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western blot analysis of lysates from wild type (WT) and HuR/ELAVL1 knockout (KO) 293T cells, using [KO Validated] HuR/ELAVL1 Rabbit pAb (P106333) at 1:1,000 dilution.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Enhanced Kit.

Exposure time: 90s.

Western blot analysis of various lysates using [KO Validated] HuR/ELAVL1 Rabbit pAb (P106333) at 1:1,000 dilution.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit.

Exposure time: 20s.

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Product Name	[KO Validated] Anti-HuR/ELAVL1 Rabbit pAb
Description	Purified Rabbit Polyclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), IF (Cell)/ICC, ELISA
Dilution	WB 1:500~1:1,000; IHC-P 1:50~1:200; IF 1:50~1:200
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Label	Unconjugated
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HuR/ELAVL1 (NP_001410.2).
Format	Affinity purified polyclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	HUR; Hua; MelG; ELAV1; HuR/ELAVL1.
Molecular weight	Calculated MW: 36 kDa; Observed MW: 36 kDa
Uniprot ID	Q15717
Gene ID	1994
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-HuR/ELAVL1 Rabbit pAb is for research use only and not for use in diagnostic or therapeutic procedures.

Background	The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy.

Background

The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy.

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P106333 [KO Validated] Anti-HuR/ELAVL1 Rabbit pAb

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