Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using GSK3β Rabbit pAb (P105100) at dilution of 1:200 (40× lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using GSK3β Rabbit pAb (P105100) at dilution of 1:200 (40× lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using GSK3β Rabbit pAb (P105100) at dilution of 1:200 (40× lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunofluorescence analysis of C6 cells using GSK3β Rabbit pAb (P105100) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of various lysates using GSK3β Rabbit pAb (P105100) at 1:1,000 dilution.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit.

Exposure time: 3s.

Western blot analysis of lysates from 293T cells, using GSK3β Rabbit pAb (P105100) at 1:1,000 dilution.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit.

Exposure time: 90s.

Western blot analysis of lysates from mouse brain, using GSK3β Rabbit pAb (P105100) at 1:1,000 dilution.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit.

Exposure time: 90s.

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg GSK3β antibody (P105100). Western blot was performed from the immunoprecipitate using GSK3β antibody (P105100) at a dilution of 1:1000.