[KO Validated] Anti-MEK7 Rabbit pAb

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[KO Validated] Anti-MEK7 Rabbit pAb

Purified Rabbit Polyclonal Antibody

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货号	规格	价格
P105097
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Immunofluorescence analysis of C6 cells using [KO Validated] MEK7 Rabbit pAb (P105097) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using [KO Validated] MEK7 Rabbit pAb (P105097) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-3 cells using MEK7 Rabbit pAb (P105097) at dilution of 1:20 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western blot analysis of lysates from wild type (WT) and MEK7 knockout (KO) 293T cells, using [KO Validated] MEK7 Rabbit pAb (P105097) at 1:1,000 dilution.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit.

Exposure time: 3s.

Western blot analysis of various lysates using [KO Validated] MEK7 Rabbit pAb (P105097) at 1:1,000 dilution.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of lysates from Rat skeletal muscle, using MEK7 Rabbit pAb (P105097) at 1:400 dilution.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit.

Exposure time: 90s.

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说明书
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Product Name	[KO Validated] Anti-MEK7 Rabbit pAb
Description	Purified Rabbit Polyclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF (Cell)/ICC, ELISA
Dilution	WB 1:500~1:1,000; IF 1:50~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human MEK7 (NP_660186.1).
Format	Affinity purified polyclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	MEK; MKK7; JNKK2; MEK 7; MAPKK7; PRKMK7; SAPKK4; SAPKK-4.
Molecular weight	Calculated MW: 47 kDa; Observed MW: 48 kDa
Uniprot ID	O14733
Gene ID	5609
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-MEK7 Rabbit pAb is for research use only and not for use in diagnostic or therapeutic procedures.

Background	The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants.

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants.

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P105097 [KO Validated] Anti-MEK7 Rabbit pAb

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