Anti-VGLUT1 Rabbit pAb

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Anti-VGLUT1 Rabbit pAb

Purified Rabbit Polyclonal Antibody

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货号	规格	价格
P103791
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Immunofluorescence analysis of Mouse brain tissue using SLC17A7 Rabbit pAb (P103791) at a dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.

Immunofluorescence analysis of Human brain tissue using SLC17A7 Rabbit pAb (P103791) at a dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.

Western blot analysis of various lysates using SLC17A7 Rabbit pAb (P103791) at 1:1,000 dilution incubated at room temperature for 1.5 hours.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25 μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit

Exposure time: 45 s.

Product Name	Anti-VGLUT1 Rabbit pAb
Description	Purified Rabbit Polyclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF (Cell)/ICC, ELISA
Dilution	WB 1:500~1:1,000; IF 1:50~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Label	Unconjugated
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human VGLUT1 (NP_064705.1).
Format	Affinity purified polyclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	BNPI; VGLUT1; SLC17A7.
Molecular weight	Calculated MW: 62 kDa; Observed MW: 55 kDa
Uniprot ID	Q9P2U7
Gene ID	57030
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-VGLUT1 Rabbit pAb is for research use only and not for use in diagnostic or therapeutic procedures.

Background	The protein encoded by this gene is a vesicle-bound, sodium-dependent phosphate transporter that is specifically expressed in the neuron-rich regions of the brain. It is preferentially associated with the membranes of synaptic vesicles and functions in glutamate transport. The protein shares 82% identity with the differentiation-associated Na-dependent inorganic phosphate cotransporter and they appear to form a distinct class within the Na+/Pi cotransporter family.

Background

The protein encoded by this gene is a vesicle-bound, sodium-dependent phosphate transporter that is specifically expressed in the neuron-rich regions of the brain. It is preferentially associated with the membranes of synaptic vesicles and functions in glutamate transport. The protein shares 82% identity with the differentiation-associated Na-dependent inorganic phosphate cotransporter and they appear to form a distinct class within the Na+/Pi cotransporter family.

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P103791 Anti-VGLUT1 Rabbit pAb

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