Anti-TMS1 Rabbit pAb

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Anti-TMS1 Rabbit pAb

Purified Rabbit Polyclonal Antibody

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货号	规格	价格
P103605
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using ASC/TMS1 Rabbit pAb (P103605) at dilution of 1:200 (40× lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil using ASC/TMS1 Rabbit pAb (P103605) at dilution of 1:200 (40× lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunofluorescence analysis of C6 cells using ASC/TMS1 Rabbit pAb (P103605) at dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using ASC/TMS1 Rabbit pAb (P103605) at dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using ASC/TMS1 Rabbit pAb (P103605) at dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western blot analysis of various lysates using ASC/TMS1 Rabbit pAb (P103605) at 1:3,000 dilution.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit.

Exposure time: 10s.

Immunoprecipitation analysis of 200 μg extracts of Rat lung using 3 μg ASC/TMS1 antibody (P103605). Western blot was performed from the immunoprecipitate using ASC/TMS1 antibody (P103605) at a dilution of 1:1000.

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Product Name	Anti-TMS1 Rabbit pAb
Description	Purified Rabbit Polyclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), IF (Cell)/ICC, IP, ELISA
Dilution	WB 1:1,000~1:6,000; IHC-P 1:50~1:200; IF 1:50~1:200; IP 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-91 of human ASC/TMS1 (NP_037390.2).
Format	Affinity purified polyclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	ASC; TMS; TMS1; CARD5; TMS-1; ASC/TMS1.
Molecular weight	Calculated MW: 22 kDa; Observed MW: 19-25 kDa
Uniprot ID	Q9ULZ3
Gene ID	29108
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-TMS1 Rabbit pAb is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.

Background

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.

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P103605 Anti-TMS1 Rabbit pAb

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