Anti-N6-methyladenosine/m6A Rabbit pAb

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Anti-N6-methyladenosine/m6A Rabbit pAb

Purified Rabbit Polyclonal Antibody

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货号	规格	价格
P103598
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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The m6A rabbit polyclonal antibody (4µg, P103598) are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer).

Oligomer 1 - N6-methyladenosine (m6A-CUGGUAACGAAUGGCU-G-Biotin)

Oligomer 2 - unmodified adenosine (ACUGGUAACGAAUGGCU-G-Biotin)

Oligomer 3 - unmodified adenosine (AAAAAAAAAAAAAAAAA-Biotin)

The m6A rabbit polyclonal antibody (500ng/100ng, P103598) are tested in Dot Blot against total RNA of Arabidopsis.

(This image is courtesy of an anonymous Abreview)

The m6A rabbit polyclonal antibody (P103598) are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine.

Oligomer 8 - ATAACTGG-m6A-CCGAATGG

Oligomer 9 - ATAACTGGACCGAATGG

Oligomer 10 - AAAAAAAAAAAAAAAA-biotin.

U2OS cells pre-treated with BrdU were subjected UVC irradiation incubated at 37 °C for the indicated time, and stained for m6A Polyclonal Antibody (P103598). DAPI, 4′,6-diamidino-2-phenylindole. Global UVC irradiation exceed cytoplasmic leavel,peaking at 2 min after irradiation and diminishing over the following 8 min.

Immunoprecipitation analysis of 25μg total RNA extracts of Arabidopsis (25μg total RNA extracts of Arabidopsis was diluted to 200 µl IP buffer system) using 2ul m6A antibody (P103598).

Product Name	Anti-N6-methyladenosine/m6A Rabbit pAb
Description	Purified Rabbit Polyclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	DB, IF (Cell)/ICC, ELISA, meRIP, Nucleotide Array
Dilution	DB 1:500~1:2,000; IF 1:50~1:200; meRIP 1:50~1:200
Reactivity	Species independent
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Label	Unconjugated
Immunogen	Chemical compounds corresponding to N6-methyladenosine / m6A.
Format	Affinity purified polyclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	N6-methyladenosine; m6A; N6-methyladenosine / m6A.
Molecular weight	0
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-N6-methyladenosine/m6A Rabbit pAb is for research use only and not for use in diagnostic or therapeutic procedures.

Background	Discovered in the 1970s, m6A is the most prevalent internal modification in polyadenylated mRNAs and long non-coding RNAs (lncRNAs) in higher eukaryotes. m6A is widely conserved among eukaryotic species that range from yeast, plants, flies to mammals, as well as among viral RNAs with a nuclear phase. The m6A-based modification is associated with a well-defined RNA motif, RRACH (R: A/G, H: A/C/U). As a representative of the epitranscriptome, m6A mRNA modifications participate in many vital activities in the cell, including stem cell self-renewal and differentiation, mRNA transcription, alternative splicing, nuclear export, translation, degradation, and microRNA processing. These processes determine the expression or inactivation of specific genes, which is vital for growth and development.(PMID: 30416848; PMID: 24662220; PMID: 30429466)

Background

Discovered in the 1970s, m6A is the most prevalent internal modification in polyadenylated mRNAs and long non-coding RNAs (lncRNAs) in higher eukaryotes. m6A is widely conserved among eukaryotic species that range from yeast, plants, flies to mammals, as well as among viral RNAs with a nuclear phase. The m6A-based modification is associated with a well-defined RNA motif, RRACH (R: A/G, H: A/C/U). As a representative of the epitranscriptome, m6A mRNA modifications participate in many vital activities in the cell, including stem cell self-renewal and differentiation, mRNA transcription, alternative splicing, nuclear export, translation, degradation, and microRNA processing. These processes determine the expression or inactivation of specific genes, which is vital for growth and development.(PMID: 30416848; PMID: 24662220; PMID: 30429466)

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P103598 Anti-N6-methyladenosine/m6A Rabbit pAb

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