Anti-Acetyl Coenzyme A Carboxylase Rabbit pAb

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Anti-Acetyl Coenzyme A Carboxylase Rabbit pAb

Purified Rabbit Polyclonal Antibody

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货号	规格	价格
P102894
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Anti-Acetyl Coenzyme A Carboxylase Rabbit pAb

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using ACC1 Rabbit pAb (P102894) at dilution of 1:50 (40× lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver using ACC1 Rabbit pAb (P102894) at dilution of 1:50 (40× lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunofluorescence analysis of C6 cells using ACC1 Rabbit pAb (P102894) at dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using ACC1 Rabbit pAb (P102894) at dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western blot analysis of various lysates using ACC1 Rabbit pAb (P102894) at 1:1,000 dilution.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit.

Exposure time: 30s.

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg ACC1 antibody (P102894). Western blot was performed from the immunoprecipitate using ACC1 antibody (P102894) at a dilution of 1:1000.

Product Name	Anti-Acetyl Coenzyme A Carboxylase Rabbit pAb
Description	Purified Rabbit Polyclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), IF (Cell)/ICC, IP, ELISA
Dilution	WB 1:500~1:2,000; IHC-P 1:50~1:200; IF 1:50~1:200; IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 2266-2346 of human ACC1 (NP_942133.1).
Format	Affinity purified polyclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	ACC; ACAC; ACC1; ACCA; Acac1; hACC1; ACACAD; ACCalpha; ACACalpha.
Molecular weight	Calculated MW: 266 kDa; Observed MW: 240 kDa
Uniprot ID	Q13085
Gene ID	31
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Acetyl Coenzyme A Carboxylase Rabbit pAb is for research use only and not for use in diagnostic or therapeutic procedures.

Background	Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.

Background

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.

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P102894 Anti-Acetyl Coenzyme A Carboxylase Rabbit pAb

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