Anti-Cyclin B1 Rabbit pAb

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Anti-Cyclin B1 Rabbit pAb

Purified Rabbit Polyclonal Antibody

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货号	规格	价格
P102893
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Immunohistochemistry analysis of paraffin-embedded Rat testis using Cyclin B1 Rabbit pAb (P102893) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cyclin B1 Rabbit pAb (P102893) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Cyclin B1 Rabbit pAb (P102893) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse testis using Cyclin B1 Rabbit pAb (P102893) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunofluorescence analysis of HeLa cells using Cyclin B1 Rabbit pAb (P102893) at dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Cyclin B1 Rabbit pAb (P102893) at dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using Cyclin B1 Rabbit pAb (P102893) at dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western blot analysis of various lysates using Cyclin B1 Rabbit pAb (P102893) at 1:500 dilution.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit.

Exposure time: 30s.

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Cyclin B1 antibody (P102893). Western blot was performed from the immunoprecipitate using Cyclin B1 antibody (P102893) at a dilution of 1:1000.

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Product Name	Anti-Cyclin B1 Rabbit pAb
Description	Purified Rabbit Polyclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), IF (Cell)/ICC, IP, ELISA
Dilution	WB 1:500~1:1,000; IHC-P 1:50~1:200; IF 1:50~1:200; IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Label	Unconjugated
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 334-433 of human Cyclin B1 (NP_114172.1).
Format	Affinity purified polyclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	CCNB 1; CCNB; ccnb1; CCNB1_HUMAN; Cyclin B1; G2 mitotic specific cyclin B1; G2/mitotic-specific cyclin-B1.
Molecular weight	Calculated MW: 48 kDa; Observed MW: 55 kDa
Uniprot ID	P14635
Gene ID	891
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Cyclin B1 Rabbit pAb is for research use only and not for use in diagnostic or therapeutic procedures.

Background	The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle.

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P102893 Anti-Cyclin B1 Rabbit pAb

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