Anti-Hsp70 Rabbit pAb

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Anti-Hsp70 Rabbit pAb

Purified Rabbit Polyclonal Antibody

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货号	规格	价格
P101323
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using HSP70 Rabbit pAb (P101323) at a dilution of 1:100 (40× lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer tissue using HSP70 Rabbit pAb (P101323) at a dilution of 1:100 (40× lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunofluorescence analysis of A-549 cells using HSP70 Rabbit pAb (P101323) at dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using HSP70 Rabbit pAb (P101323) at dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using HSP70 Rabbit pAb (P101323) at dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using HSP70 Rabbit pAb (P101323) at dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using HSP70 Rabbit pAb (P101323) at dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western blot analysis of various lysates using HSP70 Rabbit pAb (P101323) at 1:10,000 dilution incubated overnight at 4℃.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25 μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit.

Exposure time: 1s.

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Product Name	Anti-Hsp70 Rabbit pAb
Description	Purified Rabbit Polyclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), IF (Cell)/ICC, ELISA
Dilution	WB 1:1,000~1:3,000; IHC-P 1:50~1:200; IF 1:50~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Label	Unconjugated
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 500-600 of human HSP70/HSPA1 (NP_005336.3).
Format	Affinity purified polyclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	HSPA1A; HEL-S-103; HSP70-1; HSP70-1A; HSP70.1; HSP70I; HSP72; HSPA1; HSP70.
Molecular weight	Calculated MW: 70 kDa; Observed MW: 70 kDa
Uniprot ID	P0DMV8
Gene ID	3303 , 3304
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Hsp70 Rabbit pAb is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Background

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

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P101323 Anti-Hsp70 Rabbit pAb

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