Anti-NF-KB p65 Rabbit pAb

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Anti-NF-KB p65 Rabbit pAb

Purified Rabbit Polyclonal Antibody

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货号	规格	价格
P100712
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Immunofluorescence analysis of NIH/3T3 cells using NF-kB p65/RelA Rabbit pAb (P100712) at a dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using NF-kB p65/RelA Rabbit pAb (P100712) at a dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using NF-kB p65/RelA Rabbit pAb (P100712) at a dilution of 1:100 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using NF-kB p65/RelA Rabbit pAb (P100712) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of lysates from 293T cells, using NF-kB p65/RelA Rabbit pAb (P100712) at 1:1,000 dilution.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit.

Exposure time: 60s.

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg NF-kB p65/RelA antibody (P100712). Western blot was performed from the immunoprecipitate using NF-kB p65/RelA antibody (P100712) at a dilution of 1:1000.

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Product Name	Anti-NF-KB p65 Rabbit pAb
Description	Purified Rabbit Polyclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IP, IF (Cell)/ICC, ELISA
Dilution	WB 1:500~1:5000; IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells; IF 1:50~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Label	Unconjugated
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 450-551 of human NF-kB p65/RelA (NP_068810.3).
Format	Affinity purified polyclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	p65; CMCU; NFKB3; AIF3BL3; NF-kB p65/RelA.
Molecular weight	Calculated MW: 60 kDa; Observed MW: 65 kDa
Uniprot ID	Q04206
Gene ID	5970
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-NF-KB p65 Rabbit pAb is for research use only and not for use in diagnostic or therapeutic procedures.

Background	NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

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P100712 Anti-NF-KB p65 Rabbit pAb

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