Anti-DOK4 Rabbit pAb

抗体系列

Anti-DOK4 Rabbit pAb

Purified Rabbit Polyclonal Antibody

一键复制产品信息

货号	规格	价格
P100078
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

立即购买

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using DOK4 Rabbit pAb (P100078) at dilution of 1:100 (40× lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunofluorescence analysis of NIH/3T3 cells using DOK4 Rabbit pAb (P100078) at dilution of 1:50 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using DOK4 Rabbit pAb (P100078) at dilution of 1:50 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of SH-SY5Y cells using DOK4 Rabbit pAb (P100078) at dilution of 1:50 (40× lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western blot analysis of various lysates using DOK4 Rabbit pAb (P100078) at 1:1,000 dilution.

Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LF102) at 1:10,000 dilution.

Lysates/proteins: 25μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: ECL Kit.

Exposure time: 90s.

产品信息
说明书
已发表文献

Product Name	Anti-DOK4 Rabbit pAb
Description	Purified Rabbit Polyclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IF (Cell)/ICC, ELISA
Dilution	WB 1:500~1:1000; IHC-P 1:50~1:200; IF 1:50~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human DOK4 (NP_060580.2).
Format	Affinity purified polyclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	IRS5; IRS-5; DOK4.
Molecular weight	Calculated MW: 37 kDa; Observed MW: 37 kDa
Uniprot ID	Q8TEW6
Gene ID	55715
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-DOK4 Rabbit pAb is for research use only and not for use in diagnostic or therapeutic procedures.

Background	Predicted to be involved in positive regulation of MAPK cascade and transmembrane receptor protein tyrosine kinase signaling pathway. Predicted to act upstream of or within nervous system development. Predicted to be located in cytosol. Predicted to be active in cytoplasm.

说明书下载

P100078 Anti-DOK4 Rabbit pAb

总篇数：加载中...

总影响因子：加载中...

最高影响因子：加载中...

加载中...

没有更多数据

产品中心

联系雅酶

上海市闵行区新骏环路760号1号楼3层

info@epizyme.cn

400-058-8030

技术支持(电话/微信)：

罗老师 15021027255

李老师 18301721288

黄老师 18217238522

如有任何关于雅酶产品问题
欢迎来信咨询

雅酶微信公众号

抗体

蛋白提取与定量

蛋白电泳

蛋白免疫印迹

蛋白表达纯化与分离

蛋白质

ELISA试剂盒

核酸相关实验

微生物培养

设备与耗材

细胞培养

网站首页