Anti-eNOS Rabbit pAb

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Anti-eNOS Rabbit pAb

Purified Rabbit Polyclonal Antibody

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名称:Anti-eNOS Rabbit pAb\r\n货号:P011265\r\n应用:WB, ELISA\r\n稀释比:WB 1:1,000~1:2,000\r\n种属反应性:Human\r\n规格:20µL; 50µL; 100µL\r\n价格:¥588.00; ¥1080.00; ¥1780.00\r\n链接:https://www.epizyme.cn/Products/Details/P011265

货号	规格	价格
P011265
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-eNOS Rabbit pAb

All lanes: P011265 at 1:1,000 dilution

Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysates

Lane 3: U2OS (human osteosarcoma epithelial cell) whole cell lysates

Lane 4: SW620 (human colorectal carcinoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 133 kDa

Observed band size: 140 kDa

Developed using the ECL technique (Cat. No. SQ201).

产品信息
说明书

Product Name	Anti-eNOS Rabbit pAb
Description	Purified Rabbit Polyclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, ELISA
Dilution	WB 1:1,000~1:2,000
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Label	Unconjugated
Immunogen	The antiserum was produced against synthesized peptide derived from human eNOS.
Format	Affinity purified polyclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	NOS3, Nitric oxide synthase, endothelial, Constitutive NOS, cNOS, EC-NOS, Endothelial NOS, eNOS, NOS type III, NOSIII.
Calculated MW	Calculated MW: 133 kDa; Observed MW: 140 kDa
Primary Accession	P29474
Gene ID	4846
Storage	Shipped on wet ice. Store at 20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-eNOS antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Background	Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Background

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.