Anti-Notch1 Mouse mAb [61A89Q41]

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Anti-Notch1 Mouse mAb [61A89Q41]

Purified Recombinant Mouse Monoclonal Antibody

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货号	规格	价格
M900040
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-Notch1 Mouse mAb [61A89Q41]

All lanes: M900040 at 1:1,000 dilution

Lane 1: THP-1 (Human monocytic leukemia cell) whole cell lysates

Lane 2: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates

Lane 3: Rat spleen whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP Conjugated (Cat. No. LF101) at 1:5,000 dilution

Predicted band size: 272 kDa

Observed band size: 110-120 kDa

Developed using the ECL technique (Cat. No. SQ201).

Western Blot - Anti-Notch1 Mouse mAb [61A89Q41]

All lanes: M900040 at 1:1,000 dilution

Lane 1: Whole-cell lysates of 293T cells

Lane 2: Lysates from 293T cells overexpressing GFP-Flag-Notch1 (P1879-N2117)

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP Conjugated (Cat. No. LF101) at 1:5,000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

Developed using the ECL technique (Cat. No. SQ201).

Product Name	Anti-Notch1 Mouse mAb
Description	Purified Recombinant Mouse Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, ELISA
Dilution	WB 1:1,000~1:2,000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Clone No.	61A89Q41
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant protein of human Notch1
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	NOTCH1; TAN1; Neurogenic locus notch homolog protein 1; Notch 1; hN1; Translocation-associated notch protein TAN-1.
Molecular weight	Calculated MW: 272 kDa; Observed MW: 110-120 kDa
Uniprot ID	P46531
Gene ID	4851
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Notch1 Mouse mAb [61A89Q41] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma. [provided by RefSeq, Jan 2016]

Background

This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma. [provided by RefSeq, Jan 2016]

Cellular Location	Cell membrane; Single-pass type I membrane protein Late endosome membrane; Single-pass type I membrane protein Note: Non-activated receptor is targeted for lysosomal degradation via the endosomal pathway; transport from late endosomes to lysosomes requires deubiquitination by USP12. Notch 1 intracellular domain Nucleus Note: Following proteolytical processing NICD is translocated to the nucleus. Nuclear location may require MEGF10.

Cellular Location

Cell membrane; Single-pass type I membrane protein Late endosome membrane; Single-pass type I membrane protein Note: Non-activated receptor is targeted for lysosomal degradation via the endosomal pathway; transport from late endosomes to lysosomes requires deubiquitination by USP12. Notch 1 intracellular domain Nucleus Note: Following proteolytical processing NICD is translocated to the nucleus. Nuclear location may require MEGF10.

Tissue Location	In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.

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M900040 Anti-Notch1 Mouse mAb [61A89Q41]

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