Anti-STAT3 Mouse mAb [76H67N59]

抗体系列

Anti-STAT3 Mouse mAb [76H67N59]

Purified Recombinant Mouse Monoclonal Antibody

一键复制产品信息

货号	规格	价格
M900014
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

立即购买

Western Blot - Anti-STAT3 Mouse mAb [76H67N59]

All lanes: M900014 at 1:1,000 dilution

Lane 1: NIH/3T3 (Mouse Embryonic Fibroblast Cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Mouse IgG(H+L), HRP Conjugated (Cat. No. LF101) at 1:5,000 dilution

Predicted band size: 88 kDa

Observed band size: 92 kDa

Developed using the ECL technique (Cat. No. SQ201).

Western Blot - Anti-STAT3 Mouse mAb [76H67N59]

All lanes: M900014 at 1:1,000 dilution

Lane 1: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Mouse IgG(H+L), HRP Conjugated (Cat. No. LF101) at 1:5,000 dilution

Predicted band size: 88 kDa

Observed band size: 92 kDa

Developed using the ECL technique (Cat. No. SQ201).

Western Blot - Anti-STAT3 Mouse mAb [76H67N59]

All lanes: M900014 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Mouse IgG(H+L), HRP Conjugated (Cat. No. LF101) at 1:5,000 dilution

Predicted band size: 88 kDa

Observed band size: 92 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-STAT3 Mouse mAb [76H67N59]

Sample: Paraformaldehyde-fixed, paraffin embedded human endometrial carcinoma tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: M900014 at 1:200 dilution

Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunofluorescence - Anti-STAT3 Mouse mAb [76H67N59]

Sample: HeLa cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: M900014 at 1:100 dilution

Secondary antibodies: Goat anti-Mouse (488) at 1:1,000 dilution (shown in green)

Nuclei were stained with DAPI (shown in blue).

Product Name	Anti-STAT3 Mouse mAb
Description	Purified Recombinant Mouse Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), IF (Cell)/ICC, ELISA
Dilution	WB 1:1,000~1:2,000; IHC-P 1:100~1:200; IF 1:100~1:200
Reactivity	Mouse (Cell), Human
Host	Mouse
Clonality	Monoclonal
Clone No.	76H67N59
Isotype	IgG
Label	Unconjugated
Immunogen	This STAT3 antibody is generated from mice immunized with a SHC (Shrimp Hemocyanin) conjugated synthetic peptide from homo sapiens (Human).
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	1110034C02Rik, Acute Phase Response Factor, Acute-phase response factor, ADMIO, APRF, AW109958, DNA binding protein APRF, FLJ20882, HIES, MGC16063, Signal transducer and activator of transcription 3 (acute phase response factor), Signal transducer and activator of transcription 3, STAT 3, Stat3, STAT3_HUMAN.
Molecular weight	Calculated MW: 88 kDa; Observed MW: 92 kDa
Uniprot ID	P40763
Gene ID	6774
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-STAT3 Mouse mAb [76H67N59] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed:10688651, PubMed:12359225, PubMed:12873986, PubMed:15194700, PubMed:15653507, PubMed:16285960, PubMed:17344214, PubMed:18242580, PubMed:18782771, PubMed:22306293, PubMed:23084476, PubMed:28262505, PubMed:32929201). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:15653507, PubMed:16285960, PubMed:17344214, PubMed:18782771, PubMed:28262505, PubMed:32929201). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:12873986). Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes (PubMed:12359225). Activated by IL31 through IL31RA (PubMed:15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4+ T-cells into T-helper Th17 or regulatory T-cells (Treg): acetylation promotes its transcription activity and cell differentiation while deacetylation and oxidation of lysine residues by LOXL3 inhibits differentiation (PubMed:28065600, PubMed:28262505). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed:23084476). Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity). Following JAK/STAT signaling activation and as part of a complex with NFATC3 and NFATC4, binds to the alpha-beta E4 promoter region of CRYAB and activates transcription in cardiomyocytes (By similarity).

Background

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed:10688651, PubMed:12359225, PubMed:12873986, PubMed:15194700, PubMed:15653507, PubMed:16285960, PubMed:17344214, PubMed:18242580, PubMed:18782771, PubMed:22306293, PubMed:23084476, PubMed:28262505, PubMed:32929201). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:15653507, PubMed:16285960, PubMed:17344214, PubMed:18782771, PubMed:28262505, PubMed:32929201). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:12873986). Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes (PubMed:12359225). Activated by IL31 through IL31RA (PubMed:15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4+ T-cells into T-helper Th17 or regulatory T-cells (Treg): acetylation promotes its transcription activity and cell differentiation while deacetylation and oxidation of lysine residues by LOXL3 inhibits differentiation (PubMed:28065600, PubMed:28262505). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed:23084476). Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity). Following JAK/STAT signaling activation and as part of a complex with NFATC3 and NFATC4, binds to the alpha-beta E4 promoter region of CRYAB and activates transcription in cardiomyocytes (By similarity).

Cellular Location	Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.

Cellular Location

Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.

Tissue Location	Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

说明书下载

M900014 Anti-STAT3 Mouse mAb [76H67N59]

总篇数：加载中...

总影响因子：加载中...

最高影响因子：加载中...

加载中...

没有更多数据

产品中心

联系雅酶

上海市闵行区新骏环路760号1号楼3层

info@epizyme.cn

400-058-8030

技术支持(电话/微信)：

张老师 18217238522

罗老师 15021027255

李老师 18301721288

如有任何关于雅酶产品问题
欢迎来信咨询

雅酶微信公众号

抗体

蛋白提取与定量

蛋白电泳

蛋白免疫印迹

蛋白表达纯化与分离

蛋白质

ELISA试剂盒

核酸相关实验

微生物培养

设备与耗材

细胞培养

网站首页

产品中心

抗体

蛋白提取与定量

蛋白电泳

蛋白免疫印迹

蛋白表达纯化与分离

蛋白质

细胞培养

ELISA试剂盒

核酸相关实验

微生物培养

电泳成像设备与耗材

技术支持

活动资讯

联系我们

抗体系列