Flow cytometric analysis of BCL10 expression in HepG2 cells using BCL10 antibody (M020886, 1:2,000). Green, isotype control; red,BCL10 .

Immunocytochemical staining of HepG2 cells with BCL10 antibody(M020886, 1:1,000) . Nuclei were stained blue with DAPI; BCL10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using BCL10 antibody (M020886). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCL10 antibody (M020886, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using BCL10 antibody (M020886). BCL10 expression in wild-type (WT) and BCL10 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with BCL10 antibody (M020886, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of BCL10 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with BCL10 antibody (M020886, 1:2,000) and analyzed using BD flow cytometer.