Western blotting analysis using ASCC2 antibody (M020739). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ASCC2 antibody (M020739, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using ASCC2 antibody (M020739). ASCC2 expression in wild type (WT) and ASCC2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with ASCC2 antibody (M020739, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of ASCC2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ASCC2 antibody (M020739, 1:2,000) and analyzed using BD flow cytometer.

Flow cytometric analysis of ASCC2 expression in HepG2 cells using ASCC2 antibody (M020739, 1:2,000). Green, isotype control; red, ASCC2.

Immunocytochemical staining of HepG2 cells with ASCC2 antibody (M020739, 1:1,000) . Nuclei were stained blue with DAPI; ASCC2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Low. Scale bar, 20 μm.