Flow cytometric analysis of Electron transfer flavoprotein subunit alpha expression in HepG2 cells using Electron transfer flavoprotein subunit alpha antibody (M020720, 1:2,000). Green, isotype control; red, Electron transfer flavoprotein subunit alpha.

Immunocytochemical staining of HepG2 cells with Electron transfer flavoprotein subunit alpha antibody (M020720, 1:1,000) . Nuclei were stained blue with DAPI; Electron transfer flavoprotein subunit alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：High. Scale bar, 20 μm.

Western blotting analysis using electron transfer flavoprotein subunit alpha antibody (M020720). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with electron transfer flavoprotein subunit alpha antibody (M020720, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using electron transfer flavoprotein subunit alpha antibody (M020720). Electron transfer flavoprotein subunit alpha expression in wild type (WT) and electron transfer flavoprotein subunit alpha (ETFA) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with electron transfer flavoprotein subunit alpha antibody (M020720, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of Electron transfer flavoprotein subunit alpha knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Electron transfer flavoprotein subunit alpha antibody (M020720, 1:2,000) and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using Electron transfer flavoprotein subunit alpha antibody (M020720, 1:1,000), Top panel: wild-type (WT); Bottom panal: Adiponectin receptor 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Electron transfer flavoprotein subunit alpha was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.