Flow cytometric analysis of ATIC expression in C2C12 cells using ATIC antibody (M020703, 1:2,000). Green, isotype control; red, ATIC.

Western blotting analysis using ATIC antibody (M020703). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATIC antibody (M020703, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using ATIC antibody (M020703). ATIC expression in wild-type (WT) and ATIC shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATIC antibody (M020703, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of ATIC knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ATIC antibody (M020703, 1:2,000) and analyzed using BD flow cytometer.