Flow cytometric analysis of BID expression in HepG2 cells using BID antibody (M020701, 1:2,000). Green, isotype control; red, BID.

Immunocytochemical staining of HepG2 cells with BID antibody (M020701, 1:1,000) . Nuclei were stained blue with DAPI; BID was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using BID antibody (M020701). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BID antibody (M020701, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using BID antibody (M020701). BID expression in wild-type (WT) and BID shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BID antibody (M020701, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.