Western blotting analysis using BH3 interacting domain death agonist antibody (M020682). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BH3 interacting domain death agonist antibody (M020682, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using BH3 interacting domain death agonist antibody (M020682). BH3 interacting domain death agonist expression in wild-type (WT) and BH3 interacting domain death agonist (BID) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BH3 interacting domain death agonist antibody (M020682, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of BH3 interacting domain death agonist knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with BH3 interacting domain death agonist antibody (M020682, 1:2,000) and analyzed using BD flow cytometer.