[KD Validated] Anti-SMURF2 Mouse mAb [69P94M58]

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[KD Validated] Anti-SMURF2 Mouse mAb [69P94M58]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
M020672
	20µL	¥738.00
	50µL	¥1380.00
	100µL	¥2280.00

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[KD Validated] Anti-SMURF2 Mouse mAb [69P94M58]

Western blotting analysis using SMURF2 antibody (M020672). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMURF2 antibody (M020672, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit. SMURF2, SMAD specific E3 ubiquitin protein ligase 2.

Western blotting analysis using SMURF2 antibody (M020672). SMURF2 expression in wild-type (WT) and SMURF2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with SMURF2 antibody (M020672, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of SMURF2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with SMURF2 antibody (M020672, 1:2,000) and analyzed using BD flow cytometer.

Product Name	[KD Validated] Anti-SMURF2 Mouse mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC
Dilution	WB 1:1,000~1:5,000; FC 1:200~1:2,000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Clone No.	69P94M58
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant protein of human Smurf2
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	SMURF2; SMAD Specific E3 Ubiquitin Protein Ligase 2; HECT-Type E3 Ubiquitin Transferase SMURF2; SMAD Ubiquitination Regulatory Factor 2; E3 Ubiquitin-Protein Ligase SMURF2; HSMURF2; SMAD-Specific E3 Ubiquitin-Protein Ligase 2; E3 Ubiquitin Ligase SMURF2; EC 2.3.2.26; EC 6.3.2.
Molecular weight	Calculated MW: 86 kDa; Observed MW: 75 kDa
Uniprot ID	Q9HAU4
Gene ID	64750
Storage	Shipped on wet ice. Store at -20℃. Stable for 12 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	[KD Validated] Anti-SMURF2 Mouse mAb [69P94M58] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.

Background

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.

Cellular Location	Nucleus. Cytoplasm. Cell membrane. Membrane raft. Cytoplasmic in the presence of SMAD7. Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.

Tissue Location	Widely expressed.

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M020672 [KD Validated] Anti-SMURF2 Mouse mAb [69P94M58]

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