Western blotting analysis using X-linked inhibitor of apoptosis antibody (M020668). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with X-linked inhibitor of apoptosis antibody (M020668, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using X-linked inhibitor of apoptosis antibody (M020668). X-linked inhibitor of apoptosis expression in wild-type (WT) and X-linked inhibitor of apoptosis (XIAP) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with X-linked inhibitor of apoptosis antibody (M020668, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of X-linked inhibitor of apoptosis knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with X-linked inhibitor of apoptosis antibody (M020668, 1:2,000) and analyzed using BD flow cytometer.